General Cell Based Assays
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis - Economy kit (E. coli CFPS): 1 mL
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification. The 100 mL kit enables user to run 5000 reactions of 20 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C. For research use only.
Cell Biolabs Inc OxiSelect™ AOPP Assay Kit, 200 assays
Oxidative stress is defined as an increase in the production of reactive oxygen species (ROS) due to an imbalance between antioxidant and oxidants. Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of chlorinated oxidants, such as chloramines and hypochlorous acid, with plasma proteins. AOPPs are structurally similar to Advanced Glycation End-Product (AGE) proteins and exert similar biological activities. The OxiSelect™ AOPP Assay Kit offers a simple, reproducible, and consistent system for the detection of advanced oxidation protein products in plasma, lysates, and tissue homogenates. This kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.
Eurofins Discoverx PathHunter eXpress IL4R/IL13RA1 Dimerization Assay, U2OS, 10pl Kit. (93-1000E3CP5L)
The PathHunter IL4R/IL13RA1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-13) induced dimerization of IL4R/IL13RA1 receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL13RA1 target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL13RA1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human HSP27/HspB1 ASY
The R-PLEX Human HSP27 (HSPB1) Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that measures human HSP27 protein levels in serum plasma cell culture supernatants cell and tissue lysates and other biological samples This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator and controls This assay allows for the quantitative measurement of HSP27 levels with high precision and reproducibility
ACROBiosystems Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is expressed from Human, 500 tests
Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is expressed from Human
Eurofins Discoverx PathHunter eXpress GPR92 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0372E2CP6M)
The PathHunter GPR92 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of GPR92 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR92 and EA-tagged β-Arrestin-2. Activation of GPR92 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Lipid Assay Kit (neutral lipids)
Lipid Assay Kit (neutral lipids)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CXCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0248E2CP2L)
The PathHunter CXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR7 and EA-tagged β-Arrestin-2. Activation of CXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Dojindo Molecular Technologies Inc Glutamate Assay Kit-WST (100 tests) is used to determine intracellular levels of Glutamate with easy to adjust dilutions for standard curve and high stability of stock solution 100 tests
Glutamate is not only used for the biosynthesis of proteins and glutathione but also plays an important function as a neurotransmitter. Excess of glutamate is believed to contribute to neurodegenerative diseases such as Alzheimer’s disease. A recent study showed that inhibition of cystine/glutamate transporter (xCT), which takes in cystine and releases glutamate, induces ferroptosis, and current cancer research is exploring xCT as a target. Glutamate Assay Kit-WST enables quantitation of glutamate as a metabolite. This kit has been optimized to quantify glutamate in cell culture supernatants and intracellular glutamate by measuring absorbance in a colorimetric WST reaction. This kit is formatted for 96-well microplates and the assay has a detection limit of 5 μmol/l glutamate. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Cayman Chemical NAD+ (free acid), Purity: ≥90%, CAS: 53-84-9, 1 g
A signaling molecule as well as a cofactor or substrate for many enzymes
Eurofins Discoverx InCELL Hunter eXpress RIPK2 Kinase Binding Assay, 10pl Kit. (96-0050E1CP0L)
The InCELL Hunter eXpress RIPK2 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of RIPK2 via detection of RIPK2 levels. This product uses EFC technology. The cell line included overexpresses RIPK2 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0501E2CP2M)
The PathHunter TACR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. substance P, senkitide) based activation of TACR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR3 and EA-tagged β-Arrestin-2. Activation of TACR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR4 Total Internalization Assay, 10pl Kit. (93-0845E3CP27L)
The PathHunter CCR4 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR4 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CCR4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR4 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human FGFR1v3B Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human FGFR1v3B Stable Cell Line Functional Kinase Assay (U2OS) measures FGFR1 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.